Cam4 adult lk
Specifically hierarchical modelling focusing on perinatal & nutrition epidemiology & meta-analysis. MEDR5150M, Statistical modelling in health research. MSc in Epidemiology & Biostatistics: MEDR5025M, Multlevel & latent variable modelling. [email protected] 01 Summary: The development and application of statistical methods for advancing epidemiological research.
Mb Ch B: Research, Evaluation & Special Studies module 2 (RESS2).
Research, Evaluation & Special Studies module 3 (RESS3).
Extended Student-led Research or Evaluation Project (ESREP).
AML cells revealed that the levels of anti-apoptotic protein BCL2L12 were downregulated after CD82 depletion by specific short hairpin RNA (sh RNA).
Western blot analysis indicated that BCL2L12 was aberrantly expressed in patient-isolated AML cells and AML cell lines.
Furthermore, CD82 blockade by a specific antibody downregulated BCL2L12 in parallel with dephosphorylation of signal transducer and activator of transcription 5 (STAT5) and AKT, whereas pharmacological inhibition of STAT5 and AKT activation decreased BCL2L12 expression in leukemia cells.
In addition, sh RNA-mediated downregulation of BCL2L12 increased the levels of cleaved caspase-3 and suppressed proliferation of leukemia cells, impairing their engraftment in immunodeficient mice.
Taken together, our results indicate that CD82 regulated BCL2L12 expression via STAT5A and AKT signaling and stimulated proliferation and engrafting of leukemia cells, suggesting that CD82 and BCL2L12 may be promising therapeutic targets in AML.
Alterations in m RNA expression of apoptosis-related genes BCL2, BAX, FAS, caspase-3, and the novel member BCL2L12 after treatment of human leukemic cell line HL60 with the antineoplastic agent etoposide Two acute monocytic leukemia (AML-M5a) cell lines (MOLM-13 and MOLM-14) with interclonal phenotypic heterogeneity showing MLL-AF9 fusion resulting from an occult chromosome insertion, ins(11;9)(q23;p22p23)This work was supported in part by Takeda Science Foundation (to CN) and JSPS KAKENHI Grant Number 14446690 (to CN).
Author contributions Takayuki Ikezoe contributed to the concept and design, interpreted and analyzed the data and wrote the article.
Chie Nishioka performed all experiments and wrote the article.Tags: Adult Dating, affair dating, sex dating